澧炵敓鎬х幓鐠冧綋瑙嗙綉鑶滅梾鍙樼値鎬х粏鑳炲娈栬繃绋嬬殑瀹為獙鐮旂┒鐪肩鐮旂┒ 2000骞寸4鏈熺18鍗? 瀹為獙鐮旂┒浣滆?咃細鍞愬簡鏂般??鍚撮泤鑷汇??寮犳檽鍏?鍗曚綅锛?130041 闀挎槬锛岀櫧姹傛仼鍖荤澶у绗簩涓村簥瀛﹂櫌鐪肩鍏抽敭璇嶏細澧炵敓鎬х幓鐠冧綋瑙嗙綉鑶滅梾鍙橈紱缁嗚優锛涘娈栵紱瑙嗙綉鑶滆劚绂?銆?銆?鎽樿 鐩殑瑙傚療瀹為獙鎬у鐢熸?х幓鐠冧綋瑙嗙綉鑶滅梾鍙?(PVR)褰㈡垚杩囩▼涓値鎬х粏鑳炵殑澧炴畺杩囩▼銆傛柟娉曢?氳繃鐪肩悆绌垮瓟浼ゅ拰鐜荤拑浣撳唴娉ㄥ叆鑷琛?璇卞彂澶ч紶PVR妯″瀷锛屽垎鍒湪澶勭悊鍚庣1锛?3锛?7锛?14锛?21鍜?28澶╁彇妯″瀷鐪艰缁嗚優瀛﹀拰鍏嶇柅缁勫寲鏂规硶妫?娴嬬幓鐠冧綋鍜岃缃戣啘鐨勫娈栫粏鑳炪?傜粨鏋滅値鐥囧弽搴斿湪澶勭悊鍚庣1澶╁氨寮?濮嬶紝缁嗚優澧炴畺鍦ㄧ7澶╄揪楂樺嘲锛?14澶╁悗浠ュ崟鏍哥粏鑳烇紝娣嬪反缁嗚優鍜屾垚绾ょ淮缁嗚優澧炴畺涓轰富銆傚悗鏈熺幓鐠冧綋绾ょ淮鍖栵紝瑙嗙綉鑶滆劚绂伙紝缁撴瀯涓嶆竻銆傜粨璁哄疄楠屾?VR褰㈡垚杩囩▼涓紝鐐庢?х粏鑳炵殑澧炴畺瑙勫緥涓庡垱浼ゅ悗浼ゅ彛鎰堝悎杩囩▼鐩镐竴鑷淬??銆?銆?鍒嗙被鍙?銆?R?774Studies on the proliferative course of inflammatory cells in experimental PVRTang Qingxin銆?Wu Yazhen銆?Zhang Xiaoguang銆?銆?锛圖epartment of Ophthalmology锛孲econd Clinical College锛孨orman Bethune University of Medical Science锛孋hangchun 130041锛庯級銆?銆?Abstract 銆?ObjectiveTo observe the proliferative course of inflammatory cells during the development of experimental proliferative vitreoretinopathy 锛圥VR锛夛紟MethodsRat PVR models were induced by penetrating injury of eye balls and blood was injected into vitreous锛宼he model eyes were removed at different periods锛嶤ytomorphological examination was taken and immunohistochemical method was used for quantitative determination of proliferative cells on vitreous and retina锛嶳esultsThe inflammatny reaction began on d 1 after treatment and cell proliferation reached a peak on d 7锛嶢fter d 14锛宮onolytes锛宭ymphocytes and fibrocytes gradually increased锛嶸itreous fibrosis and retinal detachment or breakage were found on d 28锛嶤onclusionThe proliferative course of inflammatory cells during the development of experimental PVR is corresponding to the process of the healing course after trauma锛?銆?銆?Key words銆?proliferative vitreoretinopathy cell proliferation retinal detachment锛?銆?銆?澧炵敓鎬х幓鐠冧綋瑙嗙綉鑶滅梾鍙?(Proliferative vitreoretinopathy锛孭VR)鏄瓟婧愭?ц缃戣啘鑴辩鍜屽浼ゅ悗鐢变簬鐜荤拑浣撳強瑙嗙綉鑶滆〃闈㈢殑缁嗚優澧炴畺鍜屾敹缂╋紝閫犳垚鐗靛紩鎬ц缃戣啘鑴辩鐨勭梾鍙橈紝涔熸槸瀵艰嚧瑙嗙綉鑶滆劚绂绘墜鏈け璐ョ殑涓昏鍘熷洜銆傚ぇ閲忕爺绌跺凡缁忛槓杩颁簡PVR鑶滀腑鐨勭粏鑳炴潵婧愶紝鍏跺熀鏈殑鐥呯悊杩囩▼灏辨槸瑙嗙綉鑶滆壊绱犱笂鐨?(Pigmented retinal epithelial PRE)缁嗚優鍜岀缁忚兌璐ㄧ粏鑳炲悜鐐庢?х粏鑳炶浆鍖栵紝鍦ㄨ缃戣啘琛ㄩ潰鍜岀幓鐠冧綋鍐呮父璧般?侀檮鐫?銆佸娈栵紝褰㈡垚绾ょ淮鑶滃苟涓旀敹缂┿?備负瑙傚療PVR褰㈡垚杩囩▼涓値鎬х粏鑳炲娈栫殑鐗圭偣鍜岃寰嬶紝鎴戜滑杩涜浜嗗疄楠岀爺绌讹紝鎶ラ亾濡備笅銆?銆?銆?1 鏉愭枡涓庢柟娉?銆?銆?1锛?1 瀹為獙鍔ㄧ墿涓庡垎缁勶細Wistar澶х櫧榧?49鍙紝浣撻噸200?g卤20?g锛岄泴闆勫吋澶囷紝榧犻緞鐩歌繎銆傜敱鐧芥眰鎭╁尰绉戝ぇ瀛﹀姩鐗╅儴鎻愪緵銆傞殢鏈哄垎鎴愭甯稿鐓у拰1锛?3锛?7锛?14锛?21鍜?28澶?7缁勩??銆?銆?1锛?2 PVR鍔ㄧ墿妯″瀷鍒朵綔锛氬彇姣忓彧澶ч紶宸︾溂涓烘ā鍨嬪埗澶囩溂锛屽彸鐪间繚鐣欍??3%鎴婂反姣斿Ε0.1??mg锛弅g鑵硅厰楹婚唹銆傚湪鏃犺弻鏉′欢涓嬶紝棰炰笂鏂瑰壀寮?瑙掑珐鑶滅紭澶勭悆缁撹啘锛岃窛瑙掑珐鑶滅紭3锝?4?mm鍒嗙鎴?1锛?2鍘氬珐鑶滅摚锛屼簬鍓ョ鐨勫珐鑶滅摚涓嬫柟璺濈瑙掑珐鑶滅紭鍚?2?mm澶勫噯纭湴鍚戠幓鐠冧綋鑵斾腑蹇冮儴娉ㄥ叆榧犺嚜韬姉鍑濊20?渭l銆傚啀浜庤宸╄啘缂樺悗2?mm澶勶紝绌垮埡宸╄啘闀?1.5?mm锛屽埡鍏ユ繁搴︿负2.0?mm锛岀粨鑶滅摚瑕嗙洊锛岀粨鑶滃泭鍐呮秱鍏ユ姉鐢熺礌鐪艰啅銆?銆?銆?1锛?3 鏍囨湰鍒跺锛氬垎鍒簬鏈悗1锛?3锛?7锛?14锛?21鍜?28澶╁皢妯″瀷鐪煎拰瀵圭収缁勭溂鐞冩憳闄わ紝鍥哄畾鍖呭煁,4?渭m鍒囩墖锛孒E鏌撹壊锛屽厜闀滆瀵熸爣鏈殑鐜荤拑浣撱?佽缃戣啘鍚勫眰缁撴瀯鍜岀粏鑳炲娈栨儏鍐点?傚鐢熸?ф牳蹇冩姉鍘?(Proliferatinf cell nuclear antigen PCNA)鍏嶇柅缁勫寲鏌撹壊锛屽畾閲忓垎鏋愮値鎬х粏鑳炲娈栨儏鍐碉紝鍏惰鏁版柟娉曢噰鐢–IMA-400鍥惧儚鍒嗘瀽浠??銆?銆?1锛?4 缁熻瀛﹀鐞嗭細搴旂敤鍗曞洜绱犲绉嶆瘮杈冪▼搴?(mt)澶勭悊銆傝绋嬪簭鏉ユ簮浜庣櫧姹傛仼鍖荤澶у搴旂敤鍩虹鐮旂┒鎵?鑷紪鐨勭粺璁″绋嬪簭(DOSP)銆?銆?銆?2 缁撴灉銆?銆?2锛?1 HE鏌撹壊鍏夐暅瑙傚療锛氬鐓х粍榧犵溂鐜荤拑浣撳潎璐紝瑙嗙綉鑶滃悇灞傜粏鑳炵粨鏋勬竻鏅般??1澶╃粍鐜荤拑浣撳唴鍏呮弧娉ㄥ叆鐨勭孩缁嗚優锛岃缃戣啘琛ㄩ潰鏈夊皯閲忕櫧缁嗚優鍑虹幇锛屼富瑕佽〃鐜颁负澶ч噺绾ょ淮铔嬬櫧娓楀嚭銆?3澶╃粍瑙佹敞鍏ョ殑绾㈢粏鑳炲舰鎬佸皻瀹屾暣锛屾湁澶ч噺娴嗘恫鎬у拰绾ょ淮绱犳?ф笚鍑恒?傜幓鐠冧綋鍐呭彲瑙佽緝澶氶噺鐨勪腑鎬х矑缁嗚優锛屼笖瑙佷竴妯″瀷鐪艰缃戣啘绁炵粡涓婄毊灞備笌鑹茬礌涓婄毊灞傝劚绂汇??7澶╃粍鐐庣棁鍙嶅簲鏈?涓ラ噸锛岀幓鐠冧綋鍐呯孩缁嗚優瑁傝В锛岃鐐庢?х粏鑳炲彇浠ｃ?傜値鎬х粏鑳炰互涓?х矑缁嗚優涓轰富锛屽崟鏍哥粏鑳炴暟閲忓紑濮嬪鍔狅紝骞跺嚭鐜版垚绾ょ淮缁嗚優銆傝缃戣啘鑴辩3鍙溂(42.8%)锛岃劚绂荤殑瑙嗙綉鑶滅粍缁囩粨鏋勪笉娓呫??14澶╃粍涓?х矑缁嗚優鏄庢樉鍑忓皯锛屽崟鏍哥粏鑳炪?佹穻宸寸粏鑳炲鍔狅紝鎴愮氦缁寸粏鑳炲娈栵紝褰㈡垚鑳跺師銆?21澶╃粍瑙佸崟鏍哥粏鑳炪?佹垚绾ょ淮缁嗚優澶ч噺澧炴畺锛岃兌鍘熷鍔犲舰鎴愮槩鐥曘?傝缃戣啘澶ч儴鍒嗚劚绂伙紝瑙嗙綉鑶滃悇灞傜粨鏋勮绾ょ淮缁撶紨缁勭粐鍙栦唬銆傜幓鐠冧綋鑵斿強瑙嗙綉鑶滆〃闈㈠彲瑙佽緝澶氬惈閾佽绾㈢礌鐨勫法鍣粏鑳炪??28澶╃粍鐪肩悆钀庣缉鍙樺皬锛岀幓鐠冧綋鑵斿唴鎴愮氦缁寸粏鑳炶繃搴﹀娈栧舰鎴愮氦缁村寲锛岃缃戣啘鐮存崯锛岀粨鏋勪笉娓呫??銆?銆?2锛?2 PCNA鍏嶇柅缁勫寲瀹氶噺鍒嗘瀽锛氬簲鐢―OSP缁熻瀛︾▼搴忚瀵圭収缁勪笌鍚勭粍闂存瘮杈?(Dunnett娉?)鍜屽悇澶勭悊缁勪箣闂存瘮杈?(Newman-keu锛弒娉?)銆傜粨鏋滆〃鏄庯紝鎵嬫湳鍚?1澶╋紝鐜荤拑浣撹缃戣啘鐐庢?х粏鑳炲娈栧氨寮?濮嬨??3澶╃値鎬х粏鑳炲娈栨槑鏄撅紝鏈悗7澶╄揪楂樺嘲銆備互鍚庣値鎬х粏鑳炲娈栭?熷害鍑忔參锛岃?屼互绾ょ淮鑳跺師缁勭粐澧炴畺涓轰富锛圥锛?0.01锛夈?傛湳鍚?21澶╃殑澧炴畺閫熷害涓庢湳鍚?1澶╃浉杩戯紙P锛?0.05锛夈?傛湳鍚?28澶╋紝宸叉棤涓庢ā鍨嬪埗浣滄湁鍏崇殑鐐庢?х粏鑳炲娈栧彂鐢熴??銆?銆?3 璁ㄨ銆?銆?3锛?1 PVR鍙戠敓鍦ㄨ瀛旀簮鎬ц缃戣啘鑴辩锛岃缃戣啘澶嶄綅鎵嬫湳鍚庢垨鐪煎浼ょ瓑鎯呭喌涓嬨?傛墜鏈悗PVR鐨勫舰鎴愪富瑕佸嵄闄╁洜绱犲寘鎷湳鍓峆VR鐨勫瓨鍦ㄦ垨鑴夌粶鑶滆劚绂伙紝鐜荤拑浣撳垏鍓叉湳鐨勫簲鐢ㄦ垨澶ч潰绉殑瑙嗙綉鑶滃喎鍑?锛?1锛?銆備粠鏈川涓婅锛孭VR鐨勫舰鎴愯繃绋嬫槸缁勭粐瀵规崯浼ょ殑涓?绉嶄慨澶嶅弽搴斻?傚彂鐢熷湪浜轰綋鍏朵粬閮ㄤ綅鐨勫垱浼や慨澶嶈缁忓巻鐐庣棁鍏呭～銆佺粏鑳炲娈栧拰缁勭粐濉戝舰3涓樁娈点?侶ui绛変害灏哖VR鐨勫舰鎴愬垎涓虹値鐥囥?佺粏鑳炲娈栧拰绾ょ淮鍖栫槩鐥?3涓椂鏈?锛?2锛?銆傜敱浜庣溂缁勭粐鎹熶激鐨勭壒娈婃?э紝鐜荤拑浣撱?佽缃戣啘鐨勮В鍓栧瀹屾暣鎬у拰琛?-瑙嗙綉鑶滃睆闅滃姛鑳界殑鐮村潖锛屼娇RPE缁嗚優鏆撮湶浜庣幓鐠冧綋锛屽苟涓庡叾鎺ヨЕ銆傜爺绌惰〃鏄庯紝PVR涓殑缁濆ぇ閮ㄥ垎缁嗚優閮芥槸RPE缁嗚優鎴栬缃戣啘鑳惰川缁嗚優杞寲鑰屾潵锛孯PE鍜岃缃戣啘鑳惰川缁嗚優鑳藉憟鐜板嚭鍦ㄨ缃戣啘鍓嶈啘涓湅鍒扮殑鎵?鏈夌粏鑳炶〃鍨?锛?3锛?銆傛湰鐮旂┒瑙傚療浜嗗ぇ榧犵溂鐜荤拑浣撹厰鍐呮敞琛?鍙婄溂澶栦激鐨凱VR鍔ㄧ墿妯″瀷涓値鎬х粏鑳烇紝鎴愮氦缁寸粏鑳炵殑澧炴畺瑙勫緥锛屼负杩涗竴姝ユ帰璁≒VR褰㈡垚鏈虹悊锛屼复搴婁笂鑽墿闃叉不PVR鍜岄?夋嫨鐜荤拑浣撳垏鍓叉墜鏈殑鏈?浣虫椂鏈烘彁渚涘疄楠屼緷鎹??銆?銆?3锛?2 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